Culley, Theresa M. .
Development of microsatellite markers in Viola pubescens (Violaceae) and comparisons to allozymes and ISSRs in a population genetic study.
Allozyme and Inter-Simple Sequence Repeat (ISSR) markers are useful for population genetic studies but they are often limited by factors such as a low number of suitable loci or dominance. Microsatellite markers offer a useful alternative because they are both hypervariable and codominant, allowing heterozygosity to be directly quantified. Using a non-radioactive method with enrichment, microsatellite markers were developed in the stemmed yellow violet, Viola pubescens. Over five markers were found to be polymorphic within and among populations. These markers were subsequently used in five Ohio populations of V. pubescens for comparison with a previous genetic investigation of the same individuals using ISSR and allozyme markers. These results indicate that microsatellite markers are useful in Viola for quantifying the genetic structure of populations. Future investigations will examine the effectiveness of these microsatellite markers in other species of Viola.
1 - University of Cincinnati, Department of Biological Sciences, 614 Rieveschl Hall, Cincinnati, Ohio, 45221-0006, USA
Presentation Type: Poster
Location: Special Event Center (Cliff Lodge)
Date: Tuesday, August 3rd, 2004
Time: 12:30 PM