Systematics Section / ASPT
Freudenstein, John , Chaudhuri, Alpana , Case, Fred W. .
Relationships among and delimitation of Sarracenia species: a nuclear DNA perspective.
Sarracenia comprises approximately 11 species of North American carnivorous plants. Uncertainty in the status of some taxa persists, a situation that is exacerbated by the high degree of crossability among species. Previous analyses have demonstrated the low degree of divergence in molecular characters among the species. The purposes of this study were to employ highly variable nuclear markers to obtain an estimate of species relationships to compare with a previously generated plastid DNA tree and to address the question of species delimitation. ISSR amplification reactions were run for 28 accessions, representing all species of Sarracenia and of Darlingtonia californica, for six primers. All reactions were replicated to test for band repeatability. Reactions were run on agarose gels and band presence/absence was scored for each accession. Data were analyzed using neighbor-joining and parsimony, which gave similar patterns. With the exception of S. alabamensis, the accessions of each species form a group. Sarracenia alabamensis was shown to be distinct from S. rubra, of which it is sometimes treated as a subspecies. Sarracenia alabamensis ssp. wherryi shows stronger affinity to S. alata than to ssp. alabamensis. Whereas previous plastid DNA data placed S. minor, S. jonesii and S. oreophila together strongly, the ISSRs place S. jonesii with S. rubra, S. minor with S. psittacina, and S. oreophila is weakly linked to S. flava. Both NJ and parsimony recover a group comprising S. alabamensis, S. alata, and S. leucophylla. The differences between the nuclear and plastid patterns suggest that plastid introgression must be considered as a possibility in this group.
1 - Ohio State University, EEOB/ Herbarium, 1315 Kinnear Rd., Columbus, Ohio, 43212, USA
2 - Cranbrook Institute of Science, Bloomfield Hills, Michigan, 48303, USA
Presentation Type: Paper
Location: Cottonwood A (Snowbird Center)
Date: Wednesday, August 4th, 2004
Time: 2:00 PM