| Abstract Detail
Systematics Section / ASPT Woods, Kristi [1], Hilu, Khidir W. [1], Wiersema, John [3], Borsch, Thomas [2]. Pattern of Variation and Systematics of Nymphaea odorata: morphological and molecular evidence. Nymphaea odorata is the most widely distributed water-lily in North America. Disagreement exists on whether this morphologically quite variable species should be split into two species or subspecies. Principal component analysis of 26 vegetative characters underscores immense variability and reflects partial segregation of populations into subsp. odorata and subsp. tuberosa. Based on analysis of variance, a new set of morphological characters is proposed to discriminate between the two subspecies. Principal coordinate and minimum spanning tree analyses of ISSRs data show subsp. odorata as a distinct entity, while subsp. tuberosa is less resolved, with some samples linked to others of subsp. odorata. The trnT-F region provided a single informative site that denotes two chloroplast haplotypes corresponding to the two subspecies. In contrast, the ITS region was more variable and resolved two clades representing the two subspecies, whereas a few individuals appeared outside these two clades. Polymorphic sites were detected in ITS, and their distribution indicate possible hybridization between the subspecies. Overall, molecular evidence supports the segregation of subsp. odorata and subsp. tuberosa, with indications of limited gene flow between them.
1 - Virginia Polytechnic Institute and State University, Department of Biology, Blacksburg, Virginia, 24061, USA 2 - Rheinische Friedrich-Wilhelms-Universität Bonn, Nees-Institut für Biodiversität der Pflanzen, Meckenheimer Allee 170, Bonn, D-53115, Germany 3 - United States Department of Agriculture, Agricultural Research Service, Building 011A, BARC-West, Beltsville, Maryland, 20705, USA
Keywords: Nymphaea North America population genetic diversity trnT-F systematics ITS.
Presentation Type: Paper Session: 2-5 Location: Cottonwood A (Snowbird Center) Date: Monday, August 2nd, 2004 Time: 9:00 AM Abstract ID:641 |