Developmental and Structural Section
Serpe, Marcelo , Pegg, Saro , Chao, Wun .
Immunolocalization of cell wall components during dormancy break in underground adventitious buds of Euphorbia esula L.
Euphorbia esula (leafy spurge) is a perennial weed that produces underground adventitious buds. These buds remain dormant until the roots are separated from aerial portions of the plant. To identify biochemical mechanisms involved in the release from dormancy, we have analyzed the distribution of various polysaccharides in dormant and non-dormant buds. Dormancy break was associated with changes in pectins. The level of a homogalacturonan epitope in pectins increased during the release from dormancy. Similarly, a (1,4)β-D-galactan epitope was absent from the apical portion of dormant buds, but it was present in all portions of non-dormant buds. In contrast to these epitopes, the other carbohydrate moieties investigated were present at similar levels in dormant and non-dormant buds. These moieties include a (1,5)α-arabinan epitope, a methyl-esterified homogalacturonan epitope, callose, and carbohydrate epitopes in arabinogalactan-proteins. Overall, our results indicate that modification of pectins and synthesis of (1,4)β-D-galactan epitopes are highly regulated through bud development. This regulation may have an important role in the control of bud growth.
1 - Boise State University, Biology, 1910 University Drive, Boise, Idaho, 83725, USA
2 - USDA-ARS, Biosciences Research Laboratory, 1605 Albrecht Blvd, Fargo, ND 58105,
Presentation Type: Poster
Location: Special Event Center (Cliff Lodge)
Date: Tuesday, August 3rd, 2004
Time: 12:30 PM