Lokuge, M A , Lute, S K , Fakhar, Faiza , Francko, D A , Wilson, K G .
Comparison of cold acclimation in Sabal palmetto: callus cultures versus whole plants.
According to the field and laboratory research conducted since 1998 at Miami University several temperate palms may be grown successfully under USDA Plant Hardiness Zone 6 conditions. We have observed that palms such as Sabal palmetto, Trachycarpus fortunei and Rhapidophyllum hystrix show significant cold acclimation in late fall or early winter. Established wild-type S. palmetto survive exposure to temperatures below 100F without significant foliar injury, and some individuals recover from subzero Fahrenheit cold. Cold acclimation in higher plants is associated with numerous changes in the regulation of the genetic system and physiology. These changes are triggered by a period of low temperature exposure and are necessary to protect critical cell structures and vital physiological processes during freezing. The plasma membrane is the primary site of injury during freezing and most of the changes are aimed at preserving the integrity of cell membranes. These changes involve up- and down-regulation of gene expression and modification of pre-existing proteins. However, much remains to be elucidated about how plants survive in low temperatures. Our present investigation is aimed at determining differences in cold acclimation expression in callus cultures versus whole plants.We are using a proteomic approach to identify the proteins that are associated with cold acclimation in S. palmetto. Initially we compared the protein profiles of leaf tissues from cold-treated and untreated plants. Proteins were extracted and separated using 2-D gel electrophoresis. After staining, two proteins have been located that may be involved in cold tolerance in S. palmetto one cold inducible and one cold repressible. We now apply the same method for callus cultures to locate proteins associated with cold acclimation. Results from the proteomic study as well as results from the ion leakage assay for freezing tolerance will be used to compare the cold acclimation in callus cultures and whole plant.
1 - Miami University, Department of Botany, 316 Pearson Hall, Oxford, Ohio, 45056, USA
ion leakage assay
Presentation Type: Poster
Location: Special Event Center (Cliff Lodge)
Date: Tuesday, August 3rd, 2004
Time: 12:30 PM