Liu, Xianan , Swire-Clark, Ginger , Baird, Wm. Vance .
Stress response of HaNAC-1, a NAC-domain protein gene from Helianthus annuus (Asteraceae).
NAC domain proteins play important roles in plant development. A partial cDNA (clone RSG15-U) that was up-regulated by high salinity, was originally cloned from seedling roots of sunflower by differential display reverse transcriptase-PCR. The full-length cDNA, 745 bp, was then cloned by rapid amplification of cDNA ends. This gene, designated HaNAC-1, is predicted to have a 615 bp ORF encoding a protein composed of 205 amino acid residues. The deduced amino acid sequence was found to have 38 to 58% identities to the products of NAC genes, such as, a senescence-related cDNA (SENU5) from tomato, the no apical meristem (nam) gene from petunia and the cup-shaped cotyledon 2 (CUC2) gene from Arabidopsis. The sunflower HaNAC-1 gene was constitutively expressed at a relatively high level in seedling roots, and lower levels in leaves and seedling shoots. Expression of HaNAC-1 was up-regulated in seedling roots and shoots when exposed to high salinity, drought or exogenous abscisic acid. These results suggest that NAC domain proteins have a role in plant environmental stress response, as well as in normal development.
1 - Colorado State University, Department of Biology, Fort Collins, Colorado, 80523-1878, U.S.A.
2 - Clemson University, Horticulture, Poole Agric. Center, Clemson, South Carolina, 29634-0319, U.S.A.
no apical meristem
Presentation Type: Poster
Location: Special Event Center (Cliff Lodge)
Date: Tuesday, August 3rd, 2004
Time: 12:30 PM